eclipse dualtec separation system  (Waters Corporation)

Journal: Biomolecules

Article Title: Intermethod Characterization of Commercially Available Extracellular Vesicles as Reference Materials

doi: 10.3390/biom16010066

Figure Lengend Snippet: Mean PSD and their uncertainties by cell line. EV PSD was measured in triplicate for each method (AF 4 , Cryo-EM, MRPS, PTA) and then combined into a mean distribution by averaging the three probabilities of each replicate for three cell lines: (( a ), left ) GFP, (( b ), left ) LNCaP, and (( c ), left ) MSC. Mean PSD for AF 4 (black), Cryo-EM (red), MRPS (green), and PTA (blue). Single measurement relative uncertainties for each independent replicate in % at each diameter (nm) for three cell lines: (( a ), center ) GFP, (( b ), center ) LNCaP, and (( c ), center ) MSC for AF 4 (black), Cryo-EM (red), MRPS (green), and PTA (blue). AF 4 single measurement relative uncertainty values are near zero. Replicate-to-replicate relative uncertainties in % at each diameter (nm) for the mean distributions of three cell lines: (( a ), right ) GFP, (( b ), right ) LNCaP, and (( c ), right ) MSC for AF 4 (black), Cryo-EM (red), MRPS (green), and PTA (blue). Cryo-EM was not performed on GFP VLPs. Cryo-EM was not performed in triplicate; a single analysis of ≈500 particles was evaluated.

Article Snippet: An Eclipse DualTec AF 4 separation system (Wyatt Technology, Santa Barbara, CA, USA) was interfaced with an Agilent HPLC system (Model 1260, Agilent Technologies, Santa Clara, CA, USA) including a UV/Vis diode array detector (Model 1260, Agilent Technologies, Santa Clara, CA, USA), a HELEOS-II multiangle light scattering instrument (HELEOS-II, Wyatt Technology, Santa Barbara, CA, USA), and a differential refractive index detector (Optilab T-Rex, Wyatt Technology, Santa Barbara, CA, USA).

Techniques: Cryo-EM Sample Prep

Journal: Biomolecules

Article Title: Intermethod Characterization of Commercially Available Extracellular Vesicles as Reference Materials

doi: 10.3390/biom16010066

Figure Lengend Snippet: Comparison of mean PSD by method. Particle PSD was measured in triplicate for each cell line (LNCaP EVs, MSC EVs, and GFP VLPs) and then combined into a mean distribution by averaging the three probabilities of each replicate using four methods: ( a ) Cryo-EM, ( b ) MRPS, ( c ) PTA, and ( d ) AF 4 . Cryo-Em was not performed on GFP VLP. Cryo-EM was not performed in triplicate; a single analysis of ≈500 particles was evaluated.

Article Snippet: An Eclipse DualTec AF 4 separation system (Wyatt Technology, Santa Barbara, CA, USA) was interfaced with an Agilent HPLC system (Model 1260, Agilent Technologies, Santa Clara, CA, USA) including a UV/Vis diode array detector (Model 1260, Agilent Technologies, Santa Clara, CA, USA), a HELEOS-II multiangle light scattering instrument (HELEOS-II, Wyatt Technology, Santa Barbara, CA, USA), and a differential refractive index detector (Optilab T-Rex, Wyatt Technology, Santa Barbara, CA, USA).

Techniques: Comparison, Cryo-EM Sample Prep